Part:BBa_K4202010
Amorphous Calcium CarbonateBinding Protein(ACCBP)(without the 16 amino acids at N terminus)
Amorphous calcium carbonate binding protein(ACCBP) is a protein that can help to form and transform amorphous calcium carbonate(ACC).
In calcium carbonate solution，the ions can automatically aggregate and form prenucleation clusters, which further form ACC through certain pathways. ACC will transform into minerals of different crystal forms. In this process, organisms mainly regulate the biological mineralization process by regulating ACC formation and transformation. ACCBP is an important protein that can help to form ACC and transform ACC into calcite at specific conditions.
To ensure that this part can express active protein, the corresponding plasmid should be transformed into the E.coli, and the protein is induced at specific conditions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 45
Illegal AgeI site found at 308 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 514
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